10th European Zebrafish Meeting 2017

A Microfluidic Device for DNA Extraction from Live Zebrafish Embryos for Rapid Genotyping


Zebrafish are extensively used by biomedical researchers as a model organism for determination of genetic and biochemical pathways, identification of basic biological mechanisms, and preclinical drug discovery. Genotyping is necessary for a variety of drug screens, for use with phenotyping, and developments in functional and knockin/knock-out genomics. However, in most situations genotyping is constrained by manual procedures, which limits throughput and requires either sacrifice of the animal or waiting until they are several months of age. Our goal was to develop a device that could be automated to rapidly, accurately, and efficiently collect genetic material sufficient for genotyping from live zebrafish embryos.

B4a11f 11795a9448e6492d8169bbb2f6568dfc