A Microfluidic Device for DNA Extraction from Live Zebrafish Embryos for Rapid Genotyping
Zebrafish are extensively used by biomedical researchers as a model organism for determination of genetic and biochemical pathways, identification of basic biological mechanisms, and preclinical drug discovery. Genotyping is necessary for a variety of drug screens, for use with phenotyping, and developments in functional and knockin/knock-out genomics. However, in most situations genotyping is constrained by manual procedures, which limits throughput and requires either sacrifice of the animal or waiting until they are several months of age. Our goal was to develop a device that could be automated to rapidly, accurately, and efficiently collect genetic material sufficient for genotyping from live zebrafish embryos.