A Microfluidic Device for DNA Extraction from Live Zebrafish Embryos for Rapid Genotyping Background Zebrafish are extensively used by biomedical researchers as a model organism for determination of genetic and biochemical pathways, identification of basic biological mechanisms, and preclinical drug discovery. Genotyping is necessary for a variety of drug screens, for use with phenotyping, and …
The clinical and largely unpredictable heterogeneity of phenotypes in patients with mitochondrial disorders demonstrates the ongoing challenges in the understanding of this semi-autonomous organelle in biology and disease. Here we present a new animal model that recapitulates key components of Leigh Syndrome, French Canadian Type (LSFC), a mitochondrial disorder that includes diagnostic liver dysfunction. LSFC …
Abstract Vanishing White Matter disease (VWM) is a severe leukodystrophy of the central nervous system caused by mutations in subunits of the eukaryotic initiation factor 2B complex (eIF2B). Current models only partially recapitulate key disease features, and pathophysiology is poorly understood. Through development and validation of zebrafish (Danio rerio) models of VWM, we demonstrate that …
The ZEG device is a device for rapidly obtaining DNA from individual live zebrafish embryos for genotyping. Enables early stage identification of mutants expanding research potential and reducing husbandry costs. High PCR success rates; many downstream applications 100% correlation between ZEG genotypes and whole embryo genotypes. B4a11f 6a9682be676f4de99bf317a65109d989
An automated system for rapid cellular extraction from live zebrafish embryos and larvae: Development and application to genotyping Abstract Zebrafish are a valuable model organism in biomedical research. Their rapid development, ability to model human diseases, utility for testing genetic variants identified from next-generation sequencing, amenity to CRISPR mutagenesis, and potential for therapeutic compound screening, …
6 “semi-closed” recirculating systems (ZebTEC and WTU systems, Tecniplast) Housing of 17,000 zebrafish ‘Danio Data’ (Fulcrum) zebrafish husbandry management software Rotifer-polyculture (Best et al., 2010), Micro-artemia (Ocean Nutrition) and dry food (Skretting) Fe997a 52a147f04759438aa3bf97ed5ed788ae
