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10th European Zebrafish Meeting 2017

A Microfluidic Device for DNA Extraction from Live Zebrafish Embryos for Rapid Genotyping Background Zebrafish are extensively used by biomedical researchers as a model organism for determination of genetic and biochemical pathways, identification of basic biological mechanisms, and preclinical drug discovery. Genotyping is necessary for a variety of drug screens, for use with phenotyping, and …

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A Genetic Model Therapy Proposes a Critical Role for Liver Dysfunction in Mitochondrial Biology and Disease 2020

The clinical and largely unpredictable heterogeneity of phenotypes in patients with mitochondrial disorders demonstrates the ongoing challenges in the understanding of this semi-autonomous organelle in biology and disease. Here we present a new animal model that recapitulates key components of Leigh Syndrome, French Canadian Type (LSFC), a mitochondrial disorder that includes diagnostic liver dysfunction. LSFC …

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Vanishing White Matter Disease Expression of Truncated EIF2B5 Activates Induced Stress Response 2020

Abstract Vanishing White Matter disease (VWM) is a severe leukodystrophy of the central nervous system caused by mutations in subunits of the eukaryotic initiation factor 2B complex (eIF2B). Current models only partially recapitulate key disease features, and pathophysiology is poorly understood. Through development and validation of zebrafish (Danio rerio) models of VWM, we demonstrate that …

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Presentaion at Zebrafish Interest Group (ZIG), University of Utah 2019

The ZEG device is a device for rapidly obtaining DNA from individual live zebrafish embryos for genotyping.  Enables early stage identification of mutants expanding research potential and reducing husbandry costs.  High PCR success rates; many downstream applications 100% correlation between ZEG genotypes and whole embryo genotypes. B4a11f 6a9682be676f4de99bf317a65109d989  

PLOS ONE Journal Publication 2018

An automated system for rapid cellular extraction from live zebrafish embryos and larvae: Development and application to genotyping Abstract Zebrafish are a valuable model organism in biomedical research. Their rapid development, ability to model human diseases, utility for testing genetic variants identified from next-generation sequencing, amenity to CRISPR mutagenesis, and potential for therapeutic compound screening, …

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Zebrafish Facility Workshop 2018

6 “semi-closed” recirculating systems (ZebTEC and WTU systems, Tecniplast) Housing of 17,000 zebrafish ‘Danio Data’ (Fulcrum) zebrafish husbandry management software Rotifer-polyculture (Best et al., 2010), Micro-artemia (Ocean Nutrition) and dry food (Skretting) Fe997a 52a147f04759438aa3bf97ed5ed788ae